XE161 XDshHA-triple pt mutant-CS2+
(Plasmid
#16779)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16779 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4100
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Vector typeXenopus Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDishevelled
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Alt nameDsh
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SpeciesX. laevis (frog)
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Mutationtriple point mutation outside of the PDZ binding domain
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Entrez Genedvl2.L (a.k.a. XELAEV_18019661mg, dishevelled, dsh, dvl, dvl2, xdd1, xdsh)
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Tag
/ Fusion Protein
- HA
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
HA tagged Xenopus Dishevelled with a triple point mutation outside of the PDZ binding domain, that does not block binding to Dapper.
Notes for Use: Linearize with either Not1 or Asp718
Reference: B. Cheyette et al. (2002). Developmental Cell 2: 449-461
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
XE161 XDshHA-triple pt mutant-CS2+ was a gift from Randall Moon (Addgene plasmid # 16779 ; http://n2t.net/addgene:16779 ; RRID:Addgene_16779) -
For your References section:
Dapper, a Dishevelled-associated antagonist of beta-catenin and JNK signaling, is required for notochord formation. Cheyette BN, Waxman JS, Miller JR, Takemaru K, Sheldahl LC, Khlebtsova N, Fox EP, Earnest T, Moon RT. Dev Cell. 2002 Apr. 2(4):449-61. 10.1016/S1534-5807(02)00140-5 PubMed 11970895