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Addgene

XE151 XDpr-CS2+
(Plasmid #16771)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16771 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcs2+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    XDpr
  • Alt name
    dapper
  • Alt name
    dpr
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    2400
  • Entrez Gene
    dact1-b (a.k.a. dpr, dpr1, dact1, frodo, hdpr1, Dapper, thyex3, dapper1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 3′ cloning site Xba1 (unknown if destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Full length Xenopus Dapper, an inhibitor of Dsh signaling, as a 2.4kb EcoR1/Xba1 fragment in pCS2+. Xba1 site is DAM methylated.

Notes for Use: Linearize with either Not1 or Asp718.

Reference: B. Cheyette et al. (2002). Developmental Cell 2: 449-461.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE151 XDpr-CS2+ was a gift from Randall Moon (Addgene plasmid # 16771 ; http://n2t.net/addgene:16771 ; RRID:Addgene_16771)

  • For your References section:

    Dapper, a Dishevelled-associated antagonist of beta-catenin and JNK signaling, is required for notochord formation. Cheyette BN, Waxman JS, Miller JR, Takemaru K, Sheldahl LC, Khlebtsova N, Fox EP, Earnest T, Moon RT. Dev Cell. 2002 Apr. 2(4):449-61. 10.1016/S1534-5807(02)00140-5 PubMed 11970895
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