BirA-Myc_N_HEN1
(Plasmid
#167708)
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PurposeExpresses HEN1 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 167708 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA5/FRT/TO
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHEN1
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Alt nameUniprot Q02575
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Alt nameENSG00000171786
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SpeciesH. sapiens (human)
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Mutationno mutations
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Entrez GeneNHLH1 (a.k.a. HEN1, NSCL, NSCL1, bHLHa35)
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Tag
/ Fusion Protein
- Myc-BirA (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BirA-Myc_N_HEN1 was a gift from Markku Varjosalo (Addgene plasmid # 167708 ; http://n2t.net/addgene:167708 ; RRID:Addgene_167708) -
For your References section:
Human transcription factor protein interaction networks. Goos H, Kinnunen M, Salokas K, Tan Z, Liu X, Yadav L, Zhang Q, Wei GH, Varjosalo M. Nat Commun. 2022 Feb 9;13(1):766. doi: 10.1038/s41467-022-28341-5. 10.1038/s41467-022-28341-5 PubMed 35140242