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XE155 XDpr-delta PDZB-CS2+
(Plasmid #16767)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16767 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcs2+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    XDpr-delta PDZB
  • Alt name
    dpr
  • Alt name
    dapper
  • Species
    X. laevis (frog)
  • Mutation
    last four amino acids deleted
  • Entrez Gene
    dact1-b (a.k.a. dpr, dpr1, dact1, frodo, hdpr1, Dapper, thyex3, dapper1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 3′ cloning site Xba1 (unknown if destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Full length Xenopus Dapper (clone XE151), with last four amino acids DELETED. Xba1 site is NOT methylated.

Notes for Use: Linearize with either Not1 or Asp718.

Reference: B. Cheyette et al. (2002). Developmental Cell 2: 449-461.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE155 XDpr-delta PDZB-CS2+ was a gift from Randall Moon (Addgene plasmid # 16767 ; http://n2t.net/addgene:16767 ; RRID:Addgene_16767)

  • For your References section:

    Dapper, a Dishevelled-associated antagonist of beta-catenin and JNK signaling, is required for notochord formation. Cheyette BN, Waxman JS, Miller JR, Takemaru K, Sheldahl LC, Khlebtsova N, Fox EP, Earnest T, Moon RT. Dev Cell. 2002 Apr. 2(4):449-61. 10.1016/S1534-5807(02)00140-5 PubMed 11970895
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