pKP995
(Plasmid
#167655)
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PurposeExpress colicinB-His6, M19C, M261C
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 167655 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepACYC184
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecba-his6 M19C, M261C
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SpeciesEscherichia coli
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Mutationcba-his6 with M19C, M261C mutations
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The parent plasmid is pKP864 in this collection. Colicin B protein expression can be induced by mitomycin C treatment in a recA+ strain, i.e. not DH5alpha (PMID: 17628143).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKP995 was a gift from Kathleen Postle (Addgene plasmid # 167655 ; http://n2t.net/addgene:167655 ; RRID:Addgene_167655)