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Addgene

pKP932
(Plasmid #167652)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167652 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACYC184
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cba-his6 M261C
  • Species
    Escherichia coli
  • Mutation
    cba-his6 with M261C mutation

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The parent plasmid is pKP864 in this collection. Colicin B protein expression can be induced by mitomycin C treatment in a recA+ strain, i.e. not DH5alpha (PMID: 17628143).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKP932 was a gift from Kathleen Postle (Addgene plasmid # 167652 ; http://n2t.net/addgene:167652 ; RRID:Addgene_167652)

  • For your References section:

    A mutation in the amino terminus of a hybrid TrpC-TonB protein relieves overproduction lethality and results in cytoplasmic accumulation. Skare JT, Roof SK, Postle K. J Bacteriol. 1989 Aug;171(8):4442-7. doi: 10.1128/jb.171.8.4442-4447.1989. 10.1128/jb.171.8.4442-4447.1989 PubMed 2546922
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