XE157 GFP-XDpr-delta PDZB-CS2+
(Plasmid #16765)

• Depositing Lab: Randall Moon
• Publication: Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4095
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: XDpr-delta PDZB
• Alt name: dapper
• Alt name: dpr
• Species: X. laevis (frog)
• Mutation: XDpr is truncated to delete the last four amino acids
• Entrez Gene: dact1-b (a.k.a. dpr, dpr1, dact1, frodo, hdpr1, Dapper, thyex3, dapper1)
• Tag / Fusion Protein:
  • gfp (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Clone XE155 with a 5' GFP tag. The XDpr is truncated to delete the last four amino acids. Xba1 site is not methylated.

Notes for Use: Linearize with either Not1 or Asp718.

Reference: B. Cheyette et al. (2002). Developmental Cell 2: 449-461.