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Addgene

pKP1627
(Plasmid #167648)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167648 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD24
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    fepA G54C, delta12-16 (TonB box)
  • Species
    Escherichia coli
  • Mutation
    fepA with G54C, delta12-16 (TonB box) mutations (Mutations are delta34-38 and G76C based on start codon)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The parent is pKP515 in this collection (PMID: 12218040)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKP1627 was a gift from Kathleen Postle (Addgene plasmid # 167648 ; http://n2t.net/addgene:167648 ; RRID:Addgene_167648)

  • For your References section:

    Identification of New In Vivo TonB-FepA Rendezvous Sites. Postle K, Kho K, Gresock M, Ghosh J, Larsen R. bioRxiv 2021.12.08.471779 10.1101/2021.12.08.471779
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