pKP1383
(Plasmid
#167639)
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PurposeExpress FepA V15C . Please note that mutation numbers are not based on start codon of insert.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 167639 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBAD24
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namefepA V15C
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SpeciesEscherichia coli
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MutationfepA with V15C mutation (Mutation is V37C based on start codon)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The parent is pKP515 in this collection (PMID: 12218040)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKP1383 was a gift from Kathleen Postle (Addgene plasmid # 167639 ; http://n2t.net/addgene:167639 ; RRID:Addgene_167639) -
For your References section:
Identification of New In Vivo TonB-FepA Rendezvous Sites. Postle K, Kho K, Gresock M, Ghosh J, Larsen R. bioRxiv 2021.12.08.471779 10.1101/2021.12.08.471779