pKP2304
(Plasmid
#167633)
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PurposeExpress TonB C18G, Q160C, F202A, W213A.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 167633 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepPro33
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametonB C18G, Q160C, F202A, W213A
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SpeciesEscherichia coli
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MutationtonB with C18G, Q160C, F202A, W213A mutations. PrpR with L398M mutation.
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Parent plasmid is pKP1362 in this collection.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKP2304 was a gift from Kathleen Postle (Addgene plasmid # 167633 ; http://n2t.net/addgene:167633 ; RRID:Addgene_167633) -
For your References section:
Identification of New In Vivo TonB-FepA Rendezvous Sites. Postle K, Kho K, Gresock M, Ghosh J, Larsen R. bioRxiv 2021.12.08.471779 10.1101/2021.12.08.471779