pKP1193
(Plasmid #167614)

• Purpose: Express TonB H20N
• Depositing Lab: Kathleen Postle
• Publication: Swayne et al J Bacteriol. 2011 Aug;193(15):3693-701. doi: 10.1128/JB.05219-11. Epub 2011 Jun 10.

Backbone

• Vector backbone: pACYC184
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tonB H20N
• Species: Escherichia coli
• Mutation: tonB with H20N mutation

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that some Genbank listings and Snapgene's ORF recognition software may add additional amino acids to the TonB ORF. Postle and Skare, 1988, PMID: 2839513 demonstrated that the first two Met residues of the open reading frame are not translated and that TonB starts with the third ATG codon. The mutations listed above are numbered based on the functional starting codon.