pKP2202
(Plasmid
#167613)
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PurposeExpress ExbD V49A, P50A
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 167613 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepPro24
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameexbD V49A, P50A
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SpeciesEscherichia coli
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MutationexbD with V49A, LP50A mutation
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKP2202 was a gift from Kathleen Postle (Addgene plasmid # 167613 ; http://n2t.net/addgene:167613 ; RRID:Addgene_167613) -
For your References section:
The Intrinsically Disordered Region of ExbD Is Required for Signal Transduction. Kopp DR, Postle K. J Bacteriol. 2020 Mar 11;202(7). pii: JB.00687-19. doi: 10.1128/JB.00687-19. Print 2020 Mar 11. 10.1128/JB.00687-19 PubMed 31932309