XE248 pCDNA3.1+zeo-VSV-KLHL12
(Plasmid #16761)

• Depositing Lab: Randall Moon
• Publication: Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Backbone

• Vector backbone: pCDNA3.1+zeo
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5015
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Bleocin (Zeocin), 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pCDNA3.1+zeo-VSV-KLHL12
• Alt name: kelch-like
• Alt name: klhl2
• Species: H. sapiens (human)
• Insert Size (bp): 1721
• Entrez Gene: KLHL12 (a.k.a. C3IP1, DKIR)
• Tag / Fusion Protein:
  • VSV (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: Not1 (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH_rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pCDNA3.1 + (zeo)- VSV-KLHL12.
Nature Cell Biology 8, 348 - 357 (2006)

Addgene QC found an M11L mutation that does not affect function.