pKP1064
(Plasmid
#167607)
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PurposeExpress ExbD D25N protein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 167607 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepPro24
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameexbD D25N
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SpeciesEscherichia coli
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MutationexbD with D25N mutation
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKP1064 was a gift from Kathleen Postle (Addgene plasmid # 167607 ; http://n2t.net/addgene:167607 ; RRID:Addgene_167607) -
For your References section:
Cytoplasmic membrane protonmotive force energizes periplasmic interactions between ExbD and TonB. Ollis AA, Manning M, Held KG, Postle K. Mol Microbiol. 2009 Aug;73(3):466-81. doi: 10.1111/j.1365-2958.2009.06785.x. Epub 2009 Jul 16. 10.1111/j.1365-2958.2009.06785.x PubMed 19627500