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Addgene

pKP1064
(Plasmid #167607)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167607 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pPro24
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    exbD D25N
  • Species
    Escherichia coli
  • Mutation
    exbD with D25N mutation

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKP1064 was a gift from Kathleen Postle (Addgene plasmid # 167607 ; http://n2t.net/addgene:167607 ; RRID:Addgene_167607)
  • For your References section:

    Cytoplasmic membrane protonmotive force energizes periplasmic interactions between ExbD and TonB. Ollis AA, Manning M, Held KG, Postle K. Mol Microbiol. 2009 Aug;73(3):466-81. doi: 10.1111/j.1365-2958.2009.06785.x. Epub 2009 Jul 16. 10.1111/j.1365-2958.2009.06785.x PubMed 19627500