pKP1963
(Plasmid
#167596)
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PurposeExpress ExbB delta(195-204),ExbD
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 167596 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBAD24
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsSelect small colonies only, bacteria may require longer incubation time.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameExbB, ExbD
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SpeciesEscherichia coli
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MutationexbB gene with in-frame deletion of codons 195-204 in pKP660 (PMID: 19627500)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKP1963 was a gift from Kathleen Postle (Addgene plasmid # 167596 ; http://n2t.net/addgene:167596 ; RRID:Addgene_167596)