pKP1693
(Plasmid
#167595)
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PurposeExpress FepA with His6 tag between residues 393 and 394 of the mature amino acid sequence . Please note that mutation numbers are not based on start codon of insert.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 167595 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepPro24
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFepA
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SpeciesEscherichia coli
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MutationHis6 tag inserted after residue 393 in the mature FepA sequence of pKP1302. (His tag is between amino acids 415-416 based on start codon)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKP1693 was a gift from Kathleen Postle (Addgene plasmid # 167595 ; http://n2t.net/addgene:167595 ; RRID:Addgene_167595) -
For your References section:
Going Outside the TonB Box: Identification of Novel FepA-TonB Interactions In Vivo. Gresock MG, Postle K. J Bacteriol. 2017 Apr 25;199(10). pii: JB.00649-16. doi: 10.1128/JB.00649-16. Print 2017 May 15. 10.1128/JB.00649-16 PubMed 28264993