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Addgene

pKP1693
(Plasmid #167595)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167595 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pPro24
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FepA
  • Species
    Escherichia coli
  • Mutation
    His6 tag inserted after residue 393 in the mature FepA sequence of pKP1302. (His tag is between amino acids 415-416 based on start codon)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKP1693 was a gift from Kathleen Postle (Addgene plasmid # 167595 ; http://n2t.net/addgene:167595 ; RRID:Addgene_167595)
  • For your References section:

    Going Outside the TonB Box: Identification of Novel FepA-TonB Interactions In Vivo. Gresock MG, Postle K. J Bacteriol. 2017 Apr 25;199(10). pii: JB.00649-16. doi: 10.1128/JB.00649-16. Print 2017 May 15. 10.1128/JB.00649-16 PubMed 28264993