Skip to main content
Addgene

pKP925
(Plasmid #167591)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167591 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBR322
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    CH483
  • Growth instructions
    CH483=pro, lac, ∆(trp, tonB, opp, galU, ana). Plasmid can also be propagated in DH5alpha.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    trpC-tonB
  • Species
    Escherichia coli
  • Mutation
    Residues 1-25 TrpC; Residues 26-31 Linker; Residues 32-259 correspond to residues 12-239(end) of TonB.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that some Genbank listings and Snapgene's ORF recognition software may add additional amino acids to the TonB ORF. Postle and Skare, 1988, PMID: 2839513 demonstrated that the first two Met residues of the open reading frame are not translated and that TonB starts with the third ATG codon. The mutations listed above are numbered based on the functional starting codon.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKP925 was a gift from Kathleen Postle (Addgene plasmid # 167591 ; http://n2t.net/addgene:167591 ; RRID:Addgene_167591)

  • For your References section:

    A mutation in the amino terminus of a hybrid TrpC-TonB protein relieves overproduction lethality and results in cytoplasmic accumulation. Skare JT, Roof SK, Postle K. J Bacteriol. 1989 Aug;171(8):4442-7. doi: 10.1128/jb.171.8.4442-4447.1989. 10.1128/jb.171.8.4442-4447.1989 PubMed 2546922
Related items: