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Addgene

XE250 pCDNA3.1 + (zeo)- VSV-KLHL12-Q405X
(Plasmid #16759)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16759 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3.1+zeo
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5015
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VSV-KLHL12-Q405X
  • Alt name
    KLHL12
  • Alt name
    kelch-like 12
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1771
  • Mutation
    Q405STOP
  • Entrez Gene
    KLHL12 (a.k.a. C3IP1, DKIR)
  • Tag / Fusion Protein
    • vsv

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH_rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pCDNA3.1 + (zeo)- VSV-KLHL12-Q405X.

Nature Cell Biology 8, 348 - 357 (2006)

Addgene Qc found an M11L point mutation that does not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE250 pCDNA3.1 + (zeo)- VSV-KLHL12-Q405X was a gift from Randall Moon (Addgene plasmid # 16759 ; http://n2t.net/addgene:16759 ; RRID:Addgene_16759)

  • For your References section:

    The KLHL12-Cullin-3 ubiquitin ligase negatively regulates the Wnt-beta-catenin pathway by targeting Dishevelled for degradation. Angers S, Thorpe CJ, Biechele TL, Goldenberg SJ, Zheng N, MacCoss MJ, Moon RT. Nat Cell Biol. 2006 Apr . 8(4):348-57. 10.1038/ncb1381 PubMed 16547521
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