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Addgene

pSICO-CPSF6-iRFP670
(Plasmid #167586)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167586 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSICO
  • Backbone manufacturer
    Addgene #11578
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CPSF6-iRFP670
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2745
  • Mutation
    WT
  • Entrez Gene
    CPSF6 (a.k.a. CFIM, CFIM68, CFIM72, HPBRII-4, HPBRII-7)
  • Tag / Fusion Protein
    • iRFP670 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSICO-CPSF6-iRFP670 was a gift from Zandrea Ambrose (Addgene plasmid # 167586 ; http://n2t.net/addgene:167586 ; RRID:Addgene_167586)

  • For your References section:

    Cytoplasmic CPSF6 Regulates HIV-1 Capsid Trafficking and Infection in a Cyclophilin A-Dependent Manner. Zhong Z, Ning J, Boggs EA, Jang S, Wallace C, Telmer C, Bruchez MP, Ahn J, Engelman AN, Zhang P, Watkins SC, Ambrose Z. mBio. 2021 Mar 23;12(2). pii: mBio.03142-20. doi: 10.1128/mBio.03142-20. 10.1128/mBio.03142-20 PubMed 33758083
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