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Addgene

pcDNA5-TO-Vpr-mRuby3-IN
(Plasmid #167584)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167584 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5
  • Backbone manufacturer
    Addgene #16340
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vpr-mRuby3-IN
  • Species
    Other
  • Insert Size (bp)
    1614
  • Mutation
    WT
  • Tag / Fusion Protein
    • Vpr-mRuby3 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-TO-Vpr-mRuby3-IN was a gift from Zandrea Ambrose (Addgene plasmid # 167584 ; http://n2t.net/addgene:167584 ; RRID:Addgene_167584)

  • For your References section:

    Cytoplasmic CPSF6 Regulates HIV-1 Capsid Trafficking and Infection in a Cyclophilin A-Dependent Manner. Zhong Z, Ning J, Boggs EA, Jang S, Wallace C, Telmer C, Bruchez MP, Ahn J, Engelman AN, Zhang P, Watkins SC, Ambrose Z. mBio. 2021 Mar 23;12(2). pii: mBio.03142-20. doi: 10.1128/mBio.03142-20. 10.1128/mBio.03142-20 PubMed 33758083
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