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Addgene

pDisplay-GRAB_ATP1.0mut-IRES-mCherry-CAAX
(Plasmid #167583)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167583 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDislay
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 6546
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ATP-insensitive sensor GRAB_ATP1.0mut (control sensor)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1983
  • Entrez Gene
    P2RY1 (a.k.a. P2Y1, SARCC)
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDisplay-GRAB_ATP1.0mut-IRES-mCherry-CAAX was a gift from Yulong Li (Addgene plasmid # 167583 ; http://n2t.net/addgene:167583 ; RRID:Addgene_167583)

  • For your References section:

    A sensitive GRAB sensor for detecting extracellular ATP in vitro and in vivo. Wu Z, He K, Chen Y, Li H, Pan S, Li B, Liu T, Xi F, Deng F, Wang H, Du J, Jing M, Li Y. Neuron. 2021 Dec 17. pii: S0896-6273(21)00988-0. doi: 10.1016/j.neuron.2021.11.027. 10.1016/j.neuron.2021.11.027 PubMed 34942116
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