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PurposeExpress the ATP sensor GRAB_ATP1.0 in neurons
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 167577 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
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Backbone manufacturerAddgene
- Backbone size w/o insert (bp) 4495
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameATP sensor GRAB_ATP1.0
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SpeciesH. sapiens (human)
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Insert Size (bp)1983
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Entrez GeneP2RY1 (a.k.a. P2Y1, SARCC)
- Promoter hSyn
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.02.24.432680v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-hsyn-GRAB_ATP1.0 was a gift from Yulong Li (Addgene plasmid # 167577 ; http://n2t.net/addgene:167577 ; RRID:Addgene_167577) -
For your References section:
A sensitive GRAB sensor for detecting extracellular ATP in vitro and in vivo. Wu Z, He K, Chen Y, Li H, Pan S, Li B, Liu T, Xi F, Deng F, Wang H, Du J, Jing M, Li Y. Neuron. 2021 Dec 17. pii: S0896-6273(21)00988-0. doi: 10.1016/j.neuron.2021.11.027. 10.1016/j.neuron.2021.11.027 PubMed 34942116