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Addgene

pMeCP2miRFP670 pc3942
(Plasmid #167568)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167568 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmiRFP670-N1
  • Backbone manufacturer
    addgene # 79987
  • Backbone size w/o insert (bp) 4960
  • Total vector size (bp) 6472
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MeCp2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1476
  • Entrez Gene
    Mecp2 (a.k.a. MECP2_e1)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site MfeI (not destroyed)
  • 5′ sequencing primer gtcagatccgctagc
  • 3′ sequencing primer caactagaatgcagtg
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMeCP2miRFP670 pc3942 was a gift from Cristina Cardoso (Addgene plasmid # 167568 ; http://n2t.net/addgene:167568 ; RRID:Addgene_167568)

  • For your References section:

    Processive DNA synthesis is associated with localized decompaction of constitutive heterochromatin at the sites of DNA replication and repair. Chagin VO, Reinhart B, Becker A, Mortusewicz O, Jost KL, Rapp A, Leonhardt H, Cardoso MC. Nucleus. 2019 Dec;10(1):231-253. doi: 10.1080/19491034.2019.1688932. 10.1080/19491034.2019.1688932 PubMed 31744372
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