pENeGFP PCNAL2 pc653
(Plasmid
#167564)
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PurposeThe GFP-PCNAL2 fusion protein contains a SV40 nuclear localization signal at the NH2 terminus followed by an enhanced mutant GFP gene that is fused to the human PCNA by a linker.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 167564 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEVRF
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameProliferating Cell Nuclear Antigen
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Alt namePCNA
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SpeciesH. sapiens (human)
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GenBank IDNM_002592.2
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Entrez GenePCNA (a.k.a. ATLD2)
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Tag
/ Fusion Protein
- GFP (N terminal on insert)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENeGFP PCNAL2 pc653 was a gift from Cristina Cardoso (Addgene plasmid # 167564 ; http://n2t.net/addgene:167564 ; RRID:Addgene_167564) -
For your References section:
Dynamics of DNA replication factories in living cells. Leonhardt H, Rahn HP, Weinzierl P, Sporbert A, Cremer T, Zink D, Cardoso MC. J Cell Biol. 2000 Apr 17;149(2):271-80. doi: 10.1083/jcb.149.2.271. 10.1083/jcb.149.2.271 PubMed 10769021
