pENeGFP PCNAL2 pc653
(Plasmid #167564)

• Purpose: The GFP-PCNAL2 fusion protein contains a SV40 nuclear localization signal at the NH2 terminus followed by an enhanced mutant GFP gene that is fused to the human PCNA by a linker.
• Depositing Lab: Cristina Cardoso
• Publication: Leonhardt et al J Cell Biol. 2000 Apr 17;149(2):271-80. doi: 10.1083/jcb.149.2.271.

Backbone

• Vector backbone: pEVRF
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Proliferating Cell Nuclear Antigen
• Alt name: PCNA
• Species: H. sapiens (human)
• GenBank ID: NM_002592.2
• Entrez Gene: PCNA (a.k.a. ATLD2)
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry