pCACTUS
(Plasmid
#167559)
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Purpose(Empty Backbone) Suicide vector with the sacB gene (as a contraselective marker), CmlR gene, and temperature sensitive ori, used to perform allelic exchange mutagenesis.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 167559 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCACTUS
- Backbone size (bp) 6201
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Vector typeSuicide cloning vector, temperature sensitive.
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Resource Information
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A portion of this plasmid was derived from a plasmid made byThis plasmid originated in Prof. Paul Manning's laboratory (retired 1998) and was made by Mr. Christopher Clark.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid is unpublished and its first use was reported by Morona et al. (1995) (DOI: 10.1128/jb.177.4.1059-1068.1995), and some details published in Doyle et al. (1998) (DOI: 10.1128/IAI.66.4.1492-1499.1998).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCACTUS was a gift from Renato Morona (Addgene plasmid # 167559 ; http://n2t.net/addgene:167559 ; RRID:Addgene_167559) -
For your References section:
Molecular, genetic, and topological characterization of O-antigen chain length regulation in Shigella flexneri. Morona R, van den Bosch L, Manning PA. J Bacteriol. 1995 Feb;177(4):1059-68. doi: 10.1128/jb.177.4.1059-1068.1995. 10.1128/jb.177.4.1059-1068.1995 PubMed 7532168
Map uploaded by the depositor.
