pRMCD28
(Plasmid
#167556)
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Purpose(Empty Backbone) Plasmid pWSK29 encoding E. coli PhoA' for making reporter fusion proteins for topology mapping
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 167556 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepWSK29
- Backbone size (bp) 5400
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Vector typeBacterial Expression
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Tag
/ Fusion Protein
- PhoA' (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRMCD28 was a gift from Renato Morona (Addgene plasmid # 167556 ; http://n2t.net/addgene:167556 ; RRID:Addgene_167556) -
For your References section:
Overexpression and topology of the Shigella flexneri O-antigen polymerase (Rfc/Wzy). Daniels C, Vindurampulle C, Morona R. Mol Microbiol. 1998 Jun;28(6):1211-22. doi: 10.1046/j.1365-2958.1998.00884.x. 10.1046/j.1365-2958.1998.00884.x PubMed 9680210
