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Addgene

pRMA154
(Plasmid #167554)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167554 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJRD215
  • Backbone size w/o insert (bp) 10200
  • Vector type
    Broad host range mobilisable

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    S. flexneri O antigen genes cloned from pPM2213 (ClaI - KpnI)
  • Species
    Shigella flexneri
  • Insert Size (bp)
    13000

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRMA154 was a gift from Renato Morona (Addgene plasmid # 167554 ; http://n2t.net/addgene:167554 ; RRID:Addgene_167554)

  • For your References section:

    Characterization of the rfc region of Shigella flexneri. Morona R, Mavris M, Fallarino A, Manning PA. J Bacteriol. 1994 Feb;176(3):733-47. doi: 10.1128/jb.176.3.733-747.1994. 10.1128/jb.176.3.733-747.1994 PubMed 7507920
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