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Addgene

pCAG-Beatrix
(Plasmid #167528)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167528 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGEN
  • Backbone size w/o insert (bp) 4775
  • Total vector size (bp) 7779
  • Vector type
    Mammalian Expression, Mouse Targeting, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Beatrix
  • Alt name
    DsRed2, GFP-P2A-Cre
  • Species
    G. gallus (chicken), O. cuniculus (rabbit); Bacteriophage P1, Aequorea victoria, Discosoma sp., Oryctolagus cuniculus
  • Insert Size (bp)
    2996
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site Not (not destroyed)
  • 5′ sequencing primer pCAG-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-Beatrix was a gift from Gian Michele Ratto (Addgene plasmid # 167528 ; http://n2t.net/addgene:167528 ; RRID:Addgene_167528)

  • For your References section:

    Modelling genetic mosaicism of neurodevelopmental disorders in vivo by a Cre-amplifying fluorescent reporter. Trovato F, Parra R, Pracucci E, Landi S, Cozzolino O, Nardi G, Cruciani F, Pillai V, Mosti L, Cwetsch AW, Cancedda L, Gritti L, Sala C, Verpelli C, Maset A, Lodovichi C, Ratto GM. Nat Commun. 2020 Dec 3;11(1):6194. doi: 10.1038/s41467-020-19864-w. 10.1038/s41467-020-19864-w PubMed 33273479
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