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Addgene

PB533_2E12LI-sfGFP
(Plasmid #167527)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167527 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PB533
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    2E12LI-scFv
  • Insert Size (bp)
    746
  • Tag / Fusion Protein
    • sfGFP

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB533_2E12LI-sfGFP was a gift from Hiroshi Kimura (Addgene plasmid # 167527 ; http://n2t.net/addgene:167527 ; RRID:Addgene_167527)

  • For your References section:

    H4K20me1 and H3K27me3 are concurrently loaded onto the inactive X chromosome but dispensable for inducing gene silencing. Tjalsma SJD, Hori M, Sato Y, Bousard A, Ohi A, Raposo AC, Roensch J, Le Saux A, Nogami J, Maehara K, Kujirai T, Handa T, Bages-Arnal S, Ohkawa Y, Kurumizaka H, da Rocha ST, Zylicz JJ, Kimura H, Heard E. EMBO Rep. 2021 Feb 19:e51989. doi: 10.15252/embr.202051989. 10.15252/embr.202051989 PubMed 33605056
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