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PurposeH4K20me1-mintbody expression vector for mammalian cells
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 167526 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePB533
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name15F11-scFv
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Insert Size (bp)752
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GenBank IDLC129890
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Tag
/ Fusion Protein
- sfGFP
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB533_15F11-sfGFP was a gift from Hiroshi Kimura (Addgene plasmid # 167526 ; http://n2t.net/addgene:167526 ; RRID:Addgene_167526) -
For your References section:
A Genetically Encoded Probe for Live-Cell Imaging of H4K20 Monomethylation. Sato Y, Kujirai T, Arai R, Asakawa H, Ohtsuki C, Horikoshi N, Yamagata K, Ueda J, Nagase T, Haraguchi T, Hiraoka Y, Kimura A, Kurumizaka H, Kimura H. J Mol Biol. 2016 Oct 9;428(20):3885-3902. doi: 10.1016/j.jmb.2016.08.010. Epub 2016 Aug 14. 10.1016/j.jmb.2016.08.010 PubMed 27534817