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XE257 pCDNA3.1(zeo)-hDsh3-truncDEP
(Plasmid #16752)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16752 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3.1+zeo
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5015
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLAG-hDsh3-trunc before DEP
  • Alt name
    dvl3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1364
  • Mutation
    truncated before DEP
  • Entrez Gene
    DVL3 (a.k.a. DRS3)
  • Tag / Fusion Protein
    • flag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Nature Cell Biology 8, 348 - 357. (2006)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE257 pCDNA3.1(zeo)-hDsh3-truncDEP was a gift from Randall Moon (Addgene plasmid # 16752 ; http://n2t.net/addgene:16752 ; RRID:Addgene_16752)

  • For your References section:

    The KLHL12-Cullin-3 ubiquitin ligase negatively regulates the Wnt-beta-catenin pathway by targeting Dishevelled for degradation. Angers S, Thorpe CJ, Biechele TL, Goldenberg SJ, Zheng N, MacCoss MJ, Moon RT. Nat Cell Biol. 2006 Apr . 8(4):348-57. 10.1038/ncb1381 PubMed 16547521
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