pFLxR4
(Plasmid #167517)

• Purpose: Broad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Carbenicillin Resistance Marker.
• Depositing Lab: Christopher Reisch
• Publication: Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 6298615. doi: 10.1093/nar/gkab496.

Backbone

• Vector backbone: pFLxR4
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: LuxR-mRFP
• Species: Synthetic
• Promoter: PLuxB

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: M13 fwd

Resource Information

• A portion of this plasmid was derived from a plasmid made by: sAJM.1506 was from Addgene plasmid #108254

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry