pKCyGe2
(Plasmid
#167515)
-
PurposeBroad-Host range expression vector, RK2 and pUC origin, CymRAM repressor, PCymRC promoter controlling GmR, Kanamycin Resistance Marker.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 167515 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepKCyGe2
-
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameCymRAM-GmR
-
SpeciesSynthetic
- Promoter PCymRC
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer M13 fwd (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made bysAJM.1506 was from Addgene plasmid #108254
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pKCyGe2 was a gift from Christopher Reisch (Addgene plasmid # 167515 ; http://n2t.net/addgene:167515 ; RRID:Addgene_167515) -
For your References section:
A plasmid toolbox for controlled gene expression across the Proteobacteria. Schuster LA, Reisch CR. Nucleic Acids Res. 2021 Jun 14. pii: 6298615. doi: 10.1093/nar/gkab496. 10.1093/nar/gkab496 PubMed 34125913