pcDNA3.1/BCR-FGFR1
(Plasmid
#167490)
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PurposeMammalian expression vector for the BCR-FGFR1 fusion gene associated with myeloid and lymphoid neoplasms
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 167490 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5427
- Total vector size (bp) 8524
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBCR-FGFR1
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SpeciesH. sapiens (human)
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Insert Size (bp)3090
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GenBank IDNM_004327.4 NM_023110.3
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Entrez GeneBCR (a.k.a. ALL, BCR1, CML, D22S11, D22S662, PHL)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRl (not destroyed)
- 3′ cloning site EcoRl (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1/BCR-FGFR1 was a gift from Nicholas Cross (Addgene plasmid # 167490 ; http://n2t.net/addgene:167490 ; RRID:Addgene_167490) -
For your References section:
The t(8;22) in chronic myeloid leukemia fuses BCR to FGFR1: transforming activity and specific inhibition of FGFR1 fusion proteins. Demiroglu A, Steer EJ, Heath C, Taylor K, Bentley M, Allen SL, Koduru P, Brody JP, Hawson G, Rodwell R, Doody ML, Carnicero F, Reiter A, Goldman JM, Melo JV, Cross NC. Blood. 2001 Dec 15;98(13):3778-83. doi: 10.1182/blood.v98.13.3778. 10.1182/blood.v98.13.3778 PubMed 11739186
