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pcDNA3.1/BCR-FGFR1
(Plasmid #167490)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167490 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5427
  • Total vector size (bp) 8524
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BCR-FGFR1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3090
  • GenBank ID
    NM_004327.4 NM_023110.3
  • Entrez Gene
    BCR (a.k.a. ALL, BCR1, CML, D22S11, D22S662, PHL)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRl (not destroyed)
  • 3′ cloning site EcoRl (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1/BCR-FGFR1 was a gift from Nicholas Cross (Addgene plasmid # 167490 ; http://n2t.net/addgene:167490 ; RRID:Addgene_167490)

  • For your References section:

    The t(8;22) in chronic myeloid leukemia fuses BCR to FGFR1: transforming activity and specific inhibition of FGFR1 fusion proteins. Demiroglu A, Steer EJ, Heath C, Taylor K, Bentley M, Allen SL, Koduru P, Brody JP, Hawson G, Rodwell R, Doody ML, Carnicero F, Reiter A, Goldman JM, Melo JV, Cross NC. Blood. 2001 Dec 15;98(13):3778-83. doi: 10.1182/blood.v98.13.3778. 10.1182/blood.v98.13.3778 PubMed 11739186
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