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Addgene

CMV-mEGFP(A206K)-paCaMKII-ShadowG
(Plasmid #167475)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167475 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Customized Vector
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mEGFP(A206K)-paCaMKII-ShadowG
  • Species
    R. norvegicus (rat), Synthetic
  • Insert Size (bp)
    6472
  • Promoter CMV
  • Tags / Fusion Proteins
    • mEGFP(A206K) (N terminal on insert)
    • ShadowG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (destroyed during cloning)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Only tested in HeLa cells, but not in neurons.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-mEGFP(A206K)-paCaMKII-ShadowG was a gift from Hideji Murakoshi (Addgene plasmid # 167475 ; http://n2t.net/addgene:167475 ; RRID:Addgene_167475)

  • For your References section:

    Photoactivatable CaMKII induces synaptic plasticity in single synapses. Shibata ACE, Ueda HH, Eto K, Onda M, Sato A, Ohba T, Nabekura J, Murakoshi H. Nat Commun. 2021 Feb 2;12(1):751. doi: 10.1038/s41467-021-21025-6. 10.1038/s41467-021-21025-6 PubMed 33531495
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