pcDNA-hDDB2-K244E-V5
(Plasmid
#167471)
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Purposeectopic expression of hDDB2 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 167471 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5550
- Total vector size (bp) 6831
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDDB2
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Alt nameUV-DDB
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SpeciesH. sapiens (human)
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Insert Size (bp)1281
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MutationK244E
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Entrez GeneDDB2 (a.k.a. DDBB, UV-DDB2, XPE)
- Promoter CMV
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Tags
/ Fusion Proteins
- V5 (C terminal on backbone)
- 6xHis (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-FWD
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byJames Ford (Stanford University School of Medicine)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA-hDDB2-K244E-V5 was a gift from Steven Artandi (Addgene plasmid # 167471 ; http://n2t.net/addgene:167471 ; RRID:Addgene_167471) -
For your References section:
A novel DDB2 mutation causes defective recognition of UV-induced DNA damages and prevalent equine squamous cell carcinoma. Chen L, Bellone RR, Wang Y, Singer-Berk M, Sugasawa K, Ford JM, Artandi SE. DNA Repair (Amst). 2021 Jan;97:103022. doi: 10.1016/j.dnarep.2020.103022. Epub 2020 Nov 12. 10.1016/j.dnarep.2020.103022 PubMed 33276309