pcDNA-hDDB2-R273H-V5
(Plasmid #167469)

• Purpose: ectopic expression of hDDB2 in mammalian cells
• Depositing Lab: Steven Artandi
• Publication: Chen et al DNA Repair (Amst). 2021 Jan;97:103022. doi: 10.1016/j.dnarep.2020.103022. Epub 2020 Nov 12.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5550
• Total vector size (bp): 6831
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DDB2
• Alt name: UV-DDB
• Species: H. sapiens (human)
• Insert Size (bp): 1281
• Mutation: R273H
• Entrez Gene: DDB2 (a.k.a. DDBB, UV-DDB2, XPE)
• Promoter: CMV
• Tags / Fusion Proteins:
  • V5 (C terminal on backbone)
  • 6xHis (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-FWD
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • pcDNA3.1 hDDB2 V5-His A.gb
• A portion of this plasmid was derived from a plasmid made by: James Ford (Stanford University School of Medicine)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry