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Addgene

pCDH-3xFLAG-GFP-puroR
(Plasmid #167463)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167463 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH
  • Backbone size w/o insert (bp) 7495
  • Total vector size (bp) 8212
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP
  • Insert Size (bp)
    717
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer CMV-FWD
  • 3′ sequencing primer EF1-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH-3xFLAG-GFP-puroR was a gift from Steven Artandi (Addgene plasmid # 167463 ; http://n2t.net/addgene:167463 ; RRID:Addgene_167463)

  • For your References section:

    An Activity Switch in Human Telomerase Based on RNA Conformation and Shaped by TCAB1. Chen L, Roake CM, Freund A, Batista PJ, Tian S, Yin YA, Gajera CR, Lin S, Lee B, Pech MF, Venteicher AS, Das R, Chang HY, Artandi SE. Cell. 2018 Jun 28;174(1):218-230.e13. doi: 10.1016/j.cell.2018.04.039. Epub 2018 May 24. 10.1016/j.cell.2018.04.039 PubMed 29804836
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