XE51 XPKC-alpha
(Plasmid
#16746)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16746 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGem2
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 2869
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Vector typeXenopus Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameXPKC-Alpha
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Alt nameprotein kinase c
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Alt nameprkc
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SpeciesX. laevis (frog)
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Entrez Geneprkca (a.k.a. MGC82897, pkcalpha, pkc-alpha)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
SmaI linearize, SP6 for sense RNA.
Notes for Use:
Ref: used in Otte and Moon, Cell 68:1021-1029, 1992.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
XE51 XPKC-alpha was a gift from Randall Moon (Addgene plasmid # 16746 ; http://n2t.net/addgene:16746 ; RRID:Addgene_16746) -
For your References section:
Protein kinase C isozymes have distinct roles in neural induction and competence in Xenopus. Otte AP, Moon RT. Cell. 1992 Mar 20;68(6):1021-9. doi: 10.1016/0092-8674(92)90074-m. 10.1016/0092-8674(92)90074-m PubMed 1547501
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/07/32/1f6ab7ac-af62-11e0-90fe-003048dd6500.gif.940x940_q85_autocrop.png)