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Addgene

XE51 XPKC-alpha
(Plasmid #16746)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16746 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGem2
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2869
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    XPKC-Alpha
  • Alt name
    protein kinase c
  • Alt name
    prkc
  • Species
    X. laevis (frog)
  • Entrez Gene
    prkca (a.k.a. MGC82897, pkcalpha, pkc-alpha)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SmaI linearize, SP6 for sense RNA.

Notes for Use:
Ref: used in Otte and Moon, Cell 68:1021-1029, 1992.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE51 XPKC-alpha was a gift from Randall Moon (Addgene plasmid # 16746 ; http://n2t.net/addgene:16746 ; RRID:Addgene_16746)
  • For your References section:

    Protein kinase C isozymes have distinct roles in neural induction and competence in Xenopus. Otte AP, Moon RT. Cell. 1992 Mar 20;68(6):1021-9. doi: 10.1016/0092-8674(92)90074-m. 10.1016/0092-8674(92)90074-m PubMed 1547501