pCDH-3xFLAG-hTCAB1-puroR
(Plasmid
#167459)
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Purpose3rd-gen lentiviral expression of FLAG-TCAB1 (WDR79) in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 167459 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDH
- Backbone size w/o insert (bp) 7495
- Total vector size (bp) 9142
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTCAB1
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Alt nameWDR79
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Alt nameWRAP53
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SpeciesH. sapiens (human)
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Insert Size (bp)1647
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GenBank IDNM_001143990.2
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Entrez GeneWRAP53 (a.k.a. DKCB3, TCAB1, WDR79)
- Promoter CMV
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Tag
/ Fusion Protein
- 3xFLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (not destroyed)
- 3′ cloning site PacI (not destroyed)
- 5′ sequencing primer CMV-FWD
- 3′ sequencing primer EF1-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDH-3xFLAG-hTCAB1-puroR was a gift from Steven Artandi (Addgene plasmid # 167459 ; http://n2t.net/addgene:167459 ; RRID:Addgene_167459) -
For your References section:
An Activity Switch in Human Telomerase Based on RNA Conformation and Shaped by TCAB1. Chen L, Roake CM, Freund A, Batista PJ, Tian S, Yin YA, Gajera CR, Lin S, Lee B, Pech MF, Venteicher AS, Das R, Chang HY, Artandi SE. Cell. 2018 Jun 28;174(1):218-230.e13. doi: 10.1016/j.cell.2018.04.039. Epub 2018 May 24. 10.1016/j.cell.2018.04.039 PubMed 29804836