pHCMM3
(Plasmid
#167454)
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PurposeA control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 167454 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneNA
- Backbone size w/o insert (bp) 5702
- Total vector size (bp) 9626
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Vector typeYeast Expression
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Selectable markersLEU2, URA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePSEC63_yeGFP_cePUP-2_URA3_TADH1
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SpeciesC. elegans (nematode), S. cerevisiae (budding yeast)
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Insert Size (bp)3924
- Promoter PSEC63
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13R
- 3′ sequencing primer M13F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHCMM3 was a gift from Hugo Cesar Medina-Munoz & Marvin Wickens (Addgene plasmid # 167454 ; http://n2t.net/addgene:167454 ; RRID:Addgene_167454) -
For your References section:
Records of RNA locations in living yeast revealed through covalent marks. Medina-Munoz HC, Lapointe CP, Porter DF, Wickens M. Proc Natl Acad Sci U S A. 2020 Sep 22;117(38):23539-23547. doi: 10.1073/pnas.1921408117. Epub 2020 Sep 9. 10.1073/pnas.1921408117 PubMed 32907940
