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XE105 XPKC alpha-myc-CS2+
(Plasmid #16745)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16745 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    XPKC-alpha-myc
  • Alt name
    prkca
  • Alt name
    protein kinase c
  • Alt name
    prkc
  • Species
    X. laevis (frog)
  • Entrez Gene
    prkca (a.k.a. MGC82897, pkcalpha, pkc-alpha)
  • Tag / Fusion Protein
    • c-myc (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert: Xenopus PKC alpha with a c-myc epitope at carboxy terminus. For transcription cut with Not 1 and use SP6.

Notes for Use:
Ref: Sheldahl et al., Current Biology 9:695-698, 1999.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE105 XPKC alpha-myc-CS2+ was a gift from Randall Moon (Addgene plasmid # 16745 ; http://n2t.net/addgene:16745 ; RRID:Addgene_16745)

  • For your References section:

    Protein kinase C is differentially stimulated by Wnt and Frizzled homologs in a G-protein-dependent manner. Sheldahl LC, Park M, Malbon CC, Moon RT. Curr Biol. 1999 Jul 1;9(13):695-8. doi: 10.1016/s0960-9822(99)80310-8. 10.1016/s0960-9822(99)80310-8 PubMed 10395542
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