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Addgene

XE118 CAMKII-T286D-CS2+
(Plasmid #16736)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16736 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcS2+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CAMKII-T286D
  • Alt name
    camK
  • Alt name
    caM kinase
  • Species
    R. norvegicus (rat)
  • Mutation
    threonine 286 mutated to aspartate
  • Entrez Gene
    Camk2a (a.k.a. PK2CDD, PKCCD)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer Sp6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Description: Constitutively active CAMKII (threonine 286 mutated to aspartate)
provided by H. Schulman (Vanderbilt) subcloned into the EcoRI site of CS2+.
Linearize with Not 1 and transcribe with SP6.

Ref: M. Kuehl et al., JBC 275:12701-12711, 2000.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE118 CAMKII-T286D-CS2+ was a gift from Randall Moon (Addgene plasmid # 16736 ; http://n2t.net/addgene:16736 ; RRID:Addgene_16736)

  • For your References section:

    Ca(2+)/calmodulin-dependent protein kinase II is stimulated by Wnt and Frizzled homologs and promotes ventral cell fates in Xenopus. Kühl M, Sheldahl LC, Malbon CC, Moon RT. J Biol Chem. 2000 Apr 28. 275(17):12701-11. 10.1074/jbc.275.17.12701 PubMed 10777564
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