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Addgene

pJIT163-AFID-3
(Plasmid #167359)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167359 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJIT163
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    A3A-Cas9-UDG-T2A-AP lyase
  • Species
    Other
  • Mutation
    WT

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJIT163-AFID-3 was a gift from Caixia Gao (Addgene plasmid # 167359 ; http://n2t.net/addgene:167359 ; RRID:Addgene_167359)

  • For your References section:

    Precise, predictable multi-nucleotide deletions in rice and wheat using APOBEC-Cas9. Wang S, Zong Y, Lin Q, Zhang H, Chai Z, Zhang D, Chen K, Qiu JL, Gao C. Nat Biotechnol. 2020 Dec;38(12):1460-1465. doi: 10.1038/s41587-020-0566-4. Epub 2020 Jun 29. 10.1038/s41587-020-0566-4 PubMed 32601432
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