Seattle_IPDA_control_29_006
(Plasmid #167352)

• Purpose: ddPCR gating control for droplets containing either env+5'pol targets OR env+3'pol targets
• Depositing Lab: Florian Hladik
• Publication: Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. doi: 10.1016/j.xcrm.2021.100243. eCollection 2021 Apr 20.

Backbone

• Vector backbone: pUCIDT Amp
• Backbone manufacturer: IDT
• Vector type: Other

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pol_env
• Species: HIV-1
• Insert Size (bp): 359

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI-HF (not destroyed)
• 3′ cloning site: KpnI-HF (destroyed during cloning)
• 5′ sequencing primer: M13
• 3′ sequencing primer: M13

Resource Information

• A portion of this plasmid was derived from a plasmid made by: based on concensus sequence from HIV-1 clade B sequences from LANL
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid has partial sequences for 2 different HIV-1 clade B genes (two targets in pol and one in env).This plasmid is made by digesting the Seattle_IPDA_control_1_001 plasmid with SbfI-HF, followed by KpnI-HF. The plasmid is ligated and purified between each digestion step. partial sequences for 3 HIV-1 clade B targets: 5'pol, 3'pol and env