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Seattle_IPDA_control_17_005
(Plasmid #167351)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167351 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUCIDT Amp
  • Backbone manufacturer
    IDT
  • Vector type
    Other

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gat_tat
  • Species
    HIV-1
  • Insert Size (bp)
    199

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NsiI (not destroyed)
  • 3′ cloning site NsiI (destroyed during cloning)
  • 5′ sequencing primer M13
  • 3′ sequencing primer M13
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    based on concensus sequence from HIV-1 clade B sequences from LANL
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid has partial sequences for 2 different HIV-1 clade B genes.This plasmid is made by digesting the Seattle_IPDA_control_6_002 plasmid with PacI followed by NsiI. The plasmid is ligated and purified between each digestion step. partial sequences for 2 HIV-1 clade B targets: gag and tat

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Seattle_IPDA_control_17_005 was a gift from Florian Hladik (Addgene plasmid # 167351 ; http://n2t.net/addgene:167351 ; RRID:Addgene_167351)

  • For your References section:

    A highly multiplexed droplet digital PCR assay to measure the intact HIV-1 proviral reservoir. Levy CN, Hughes SM, Roychoudhury P, Reeves DB, Amstuz C, Zhu H, Huang ML, Wei Y, Bull ME, Cassidy NAJ, McClure J, Frenkel LM, Stone M, Bakkour S, Wonderlich ER, Busch MP, Deeks SG, Schiffer JT, Coombs RW, Lehman DA, Jerome KR, Hladik F. Cell Rep Med. 2021 Apr 12;2(4):100243. doi: 10.1016/j.xcrm.2021.100243. eCollection 2021 Apr 20. 10.1016/j.xcrm.2021.100243 PubMed 33948574
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