Human_TFAM_dHMGA_pET28
(Plasmid #167329)

• Purpose: Expresses TFAM dHMGA mutant in bacterial cells
• Depositing Lab: Tom Misteli
• Publication: Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi: 10.15252/embj.2020107165. Epub 2021 Feb 23.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 4927
• Total vector size (bp): 5713
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TFAM
• Alt name: Mitochondrial transcription factor A
• Species: H. sapiens (human)
• Insert Size (bp): 544
• Mutation: Truncation mutant containing linker, HMGB, and C-tail domains (123-246). This mutant lacks the HMGA domain.
• GenBank ID: NM_003201.3
• Entrez Gene: TFAM (a.k.a. MTDPS15, MTTF1, MTTFA, TCF6, TCF6L1, TCF6L2, TCF6L3)
• Promoter: T7
• Tag / Fusion Protein:
  • 6XHis (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminal

Resource Information

• Supplemental Documents:
  • BAC1515_Human_TFAM_dHMGA_pET28.ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/822858v4 for BioRxiv preprint