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Addgene

Gfp-zf-vasa
(Plasmid #167323)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167323 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM Teasy vector
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3015
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    zf vasa 3'UTRs
  • Species
    D. rerio (zebrafish)
  • GenBank ID
    BC129275
  • Entrez Gene
    ddx4 (a.k.a. v, vas, vasa, vlg, wu:fi24g0, wu:fi24g05, zgc:109812, zgc:158535)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

hrGfp

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Gfp-zf-vasa was a gift from Goro Yoshizaki (Addgene plasmid # 167323 ; http://n2t.net/addgene:167323 ; RRID:Addgene_167323)

  • For your References section:

    Green fluorescent protein labeling of primordial germ cells using a nontransgenic method and its application for germ cell transplantation in salmonidae. Yoshizaki G, Tago Y, Takeuchi Y, Sawatari E, Kobayashi T, Takeuchi T. Biol Reprod. 2005 Jul;73(1):88-93. doi: 10.1095/biolreprod.104.034249. Epub 2005 Mar 2. 10.1095/biolreprod.104.034249 PubMed 15744027
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