Gfp-rt-vasa
(Plasmid
#167322)
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PurposePlasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of rainbow trout by in vitro transcription.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 167322 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEM Teasy vector
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3015
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namert vasa 3'UTRs
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Speciesrainbow trout
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GenBank IDAB032566
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Entrez Geneddx4 (a.k.a. vas)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
hrGfp
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Gfp-rt-vasa was a gift from Goro Yoshizaki (Addgene plasmid # 167322 ; http://n2t.net/addgene:167322 ; RRID:Addgene_167322) -
For your References section:
Green fluorescent protein labeling of primordial germ cells using a nontransgenic method and its application for germ cell transplantation in salmonidae. Yoshizaki G, Tago Y, Takeuchi Y, Sawatari E, Kobayashi T, Takeuchi T. Biol Reprod. 2005 Jul;73(1):88-93. doi: 10.1095/biolreprod.104.034249. Epub 2005 Mar 2. 10.1095/biolreprod.104.034249 PubMed 15744027